DNA

Part:BBa_K4051017:Design

Designed by: Clodagh Healy   Group: iGEM21_MTU-CORK   (2021-10-08)


PCRB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 342
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E.coli


Source

Dechloromonas (Azospira) suillum locus Dsui_0148

Genbank Accession: CP003153.1

Generic Histag

Generic secretion signal from Gaussian luciferase

References